KCO1 is a component of the slow-vacuolar (SV) ion channel

FEBS Letters 511: 28-32 (2002)

Schönknecht, G., Spoormaker, P., Steinmeyer, R., Brüggeman, L., Ache, P., Dutta, R., Reintanz, B., Godde, M., Hedrich, R., Palme, K.

The Arabidopsis double pore K⁺ channel KCO1 was fused to green fluorescent protein and expressed in tobacco protoplasts. Microscopic analysis revealed a bright green fluorescence at the vacuolar membrane. RT-PCR experiments showed that KCO1 is expressed in the mesophyll. Vacuoles from Arabidopsis wild-type and kco1 knockout plants were isolated for patch-clamp analyses. Currents mediated by slow-activating vacuolar (SV) channels of mesophyll cell vacuoles were significantly smaller in kco1 plants compared to the wild-type. This shows that KCO1 is involved in the formation of SV channels.