Dr. Yinghua Huang

Adjunct Professor of Botany

Adjunct Professor of Natural Resource, Ecology & Management (NREM)

Lead Scientist & Research Geneticist, USDA-ARS

Email: yinghua.huang@okstate.edu
Email: yinghua.huang@ars.usda.gov
Phone: 405-624-4141, ext. 230
Fax: 405-624-4142
Address: USDA-ARS, Plant Science Research Laboratory, 1301 N. Western Road, Stillwater OK 74075

RESEARCH:

The overall goal of my research program is to improve understanding of plant-pest interaction and host defense, thus leading to development of high-performance sorghum germplasm with durable genetic resistance to insect pests and diseases, through integrated research approaches, such as exploiting untapped plant genetic diversity in plant germplasm collections, molecular interactions between host plants and pest, profiling of gene expression, gene discovery, gene markers, and functional genomics.

Current research projects include (1) identification of new sources of genetic resistance and characterization of their resistance mechanisms, (2) profiling of global changes in gene expression during host-pest interactions and functional analysis of those differentially expressed genes, (3) molecular mapping of resistance QTLs and developing DNA markers for marker-assisted selection (MAS), and (4) development of insect resistant germplasm or cultivars using both biotechnological and conventional methods.

(http://www.ars.usda.gov/pandp/people/people.htm?personid=32552)

PUBLICATIONS:

Huang Y. 2006. Molecular defense responses to a phloem-feeding aphid in sorghum involve both general defense strategies and specific resistance mechanisms. Molecular Genetics and Genomics (under revision).

Wu, Y. and Y. Huang. 2006. Molecular mapping of QTLs for resistance to the greenbug Schizaphis graminum (Rondani) in Sorghum bicolor (Moench). Theor Appl Genet (in press).

Wang X., Y. Huang, A.J. Mort, Y. Zeng, C.G. Tauer, and K.D. Cochran (2006) Variation of Taxane content in needles of Taxus x media cultivars with different growth characteristics. Zeitschrift fur Naturforschung C (in press).

Park S-J, Y Huang, and P Ayoubi. 2006. Identification of expression profiles of sorghum genes in response to greenbug phloem-feeding using cDNA subtraction and microarray analysis. Planta 223:932:947.

Wu, Y., Y. Huang, C.G. Tauer, and D.R. Porter. 2006. Genetic diversity of sorghum accessions resistant to greenbugs as assessed with AFLP markers. Genome 49:143-149.

Huang Y. 2005. Genetic transformation of sorghum using Agrobacterium and immature inflorescences. International Sorghum and Millets Newsletter 46: 69-72.

Huang, Y. 2005. Application of functional genomics to crop pest management. Proceedings of the 24 th Biennial Sorghum Industry Conference and North American Grain Congress. pp. 62-73.

Kresovich, S., Barbazuk, B., Bedell, J., Borrell, A., Buell, R., Burke, J.J., Clifton, S., Cordonnier-Pratt, M., Cox, S., Dahlberg, J., Erpelding, J.E., Fulton, T.M., Fulton, B., Fulton, L., Gingle, A., Goff, S., Hash, C., Huang, Y., Jordan, D., Klein, P., Klein, R.R., Magalhaes, J., Mccombie, R., Moore, P.H., Mullet, J.E., Ozias-Akins, P., Paterson, A.H., Porter, K., Pratt, L., Roe, B., Rooney, W., Schnable, P., Steely, D.M., Tuinstra, M., Ware, D., Warek, U. 2005. Toward sequencing the sorghum genome: A US National Science Foundation-sponsored Workshop Report. Plant Physiology 138(4): 1898-1902.

Huang, Y. 2005. Molecular interactions between crop plants and phloem-feeding aphid. Comparative Biochemistry and Physiology, Part A 141: S229-S230.

Huang, Y. 2004. Examining plant defense responses to greenbug attack in sorghum using DNA microarray technology. International Sorghum and Millets Newsletter. 44: 72-74.

Chen, J.W., C.G. Tauer, G.H. Bai, Y. Huang, M.E. Payton, and A.G. Holley. 2004. Biodirectional genetic introgression between Pinus taeda and Pinus echinata: evidence from morphological and molecular data. Can. J. For. Res. 34: 2508-2516.

Huang, Y., N. Jin, A. Diner, C.G. Tauer, Y. Zhang, and J. Damicone. 2004. Genetic analysis of a disease resistance gene from loblolly pine. SFTIC proceedings. pp. 173-180.

Chen, J.W., C.G. Tauer, and Y. Huang. 2004. Introgression between Pinus taeda L. and Pinus echinata Mill. SFTIC proceedings. pp. 56-59.

Wu, L., S. Hallgren, Y. Huang, K. Conway, and C.G. Tauer. 2003. Storage protein mobilization and thiol protease up-regulation by solid matrix priming in loblolly pine (Pinus taeda) seed embryos. Seed Science and Technology. 31:667-680.

Chen, J, CG Tauer, & Y Huang. 2002. Nucleotide sequences of the internal transcribed spacers and 5.8S region of nuclear ribosomal DNA in Pinus taeda and Pinus echinata. DNA Sequencing – The Jopurnal of Sequencing and Mapping 13(2): 129-131.

Chen, J, CG Tauer, & Y Huang. 2002. Paternal chloroplast inheritance patterns in pine hybrids detected with trnL-trnF intergenic region polymorphism. Theor Appl Genet 104:1307-1311.

Diner, AM, A Zipf, R Ward, Y Huang & G Brown, Jr. 1999. Transient expression of GUS in bombarded embryogenic longleaf, loblolly, and Eastern white pine. USDA Forest Service Research Note SRS-7 (May 1999): 1-4.

Huang Y, CG Tauer & S Zhan. 1999. An approach to genetic engineering of taxol production. In Vitro Cellular & Developmental Biology 35 (3): 48.

Liu J, Y Huang, B Ding & CG Tauer. 1999. cDNA cloning and expression of a sweetgum gene that shows homology with Arabidopsis AGAMOUS gene. Plant Science 142:73-82.

Huang Y & CG Tauer. 1998. Genetic Transformation in Conifers. In: S. Puri (ed.), Tree Improvement: Applied Research and Technology Transfer. Oxford & IBH Co., India, pp. 301-325 (Invited Chapter)

Raja RG, CG Tauer, RF Wittwer & Y Huang. 1998. Regeneration methods affect genetic variation and structure in shortleaf pine (Pinus echinata Mill.). Forest Genetics 5 (3):171-178.

Raja RG, CG Tauer, RF Wittwer & Y Huang. 1997. Segregation and linkage relationships of isozymes in shortleaf pine (Pinus echinata Mill.). Theor Appl Genet 95(8): 1252-1257.

Hallgren SW, L Wu, Y Huang & D Ferris. 1997. Water status and protein profiles of loblolly pine seed germination enhancement by solid matrix priming. Plant Physiol. 114 (3):45-46.

Raja RG, CG Tauer, RF Wittwer & Y Huang. 1997. An isoenzymatic study of the genetic structure in natural populations of shortleaf pine, Pinus echinata Mill. Can J For Res 27(5):740-749.

Wittwer RF, CG Tauer, MM Huebschmann & Y Huang. 1996. Estimating seed quantity and quality in shortleaf pine (Pinus echinata Mill.) cones from natural stands. New Forest 14:45-53.

Huang Y & CG Tauer. 1995. Genetic transformation of southern pines: A summary of recent research. In: Wang, S. and X. Jiang (eds) Growth and Development Control and Biotechnology in Woody Plants, China Forestry Publishing House, Beijing, pp. 41-60.

Shin D, GK Podila, Y Huang & DF Karnosky. 1994. Transgenic larch expressing genes for herbicide and insect resistance. Can J Forest Res 24:2059-2067.

Huang Y & CG Tauer. 1994. Integrative transformation of Pinustaeda L. and P.echinata Mill. by Agrobacterium tumefaciens. Forest Genetics 1(1):23-31.

Ellis DD, AM Diner & Y Huang. 1993. Regeneration of the genetically engineered conifer - the importance of the biological system. In: G.S. Forter and A.M. Diner (eds.), Proc. of the Southern Regional Information Exchange Group (SRIEG) Biennial Symposium on Forest Genetics. Gen. Tech. Rep. SO-108. New Orleans, Louisiana, pp. 39-48.

Diner AM & Y Huang. 1993. Site-specific polymorphic tumorigenesis induced in single Larix decidua germlings by Agrobacterium rhizogenes. USDA For. Ser. Gen. Tech. Rep. NC-154: 48

Huang Y, DD Stokke, AM Diner, WM Barnes & DF Karnosky. 1993. Virulence of Agrobacterium on Larix decidua and their cellular Interactions as depicted by scanning electron microscopy. J Experimental Bot 44:1191-1201.

Huang Y (ed.). 1993. Genetic Engineering of Larix decidua: Agrobacterium-Mediated Gene Transfer and Regeneration of Transgenic Plants in European Larch. UMI Publishing Co., Ann Arbor, MI, 245 pp.

Huang Y, DF Karnosky & CG Tauer. 1993. Applications of biotechnology and molecular genetics to tree improvement. J Arboriculture 19(2):84-98.

Huang Y, AM Diner, & DF Karnosky. 1991. Agrobacterium rhizogenes-mediated genetic transformation and regeneration of a conifer: Larix decidua. In Vitro Cellular & Developmental Biology 27: 201-207.